Seminar: Macromolecular crystallography at the European Spallation Source

The structure determination of biological macromolecules by X-ray crystallography is a key technique in biochemistry and widely applied in the pharmaceutical industry as well. However, the positions of hydrogen atoms – often crucial for understanding the biological function – can generally not be determined by X-ray crystallography. Neutron macromolecular crystallography is the most unambiguous method for locating the hydrogens, but it remains technically far more challenging due to the low brilliance of the available neutron sources and has therefore been applied only to relatively few systems.
    The ESS long pulse spallation source is particularly well suited for macromolecular crystallography, where only modest time-of-flight resolution is required. We aim to routinely resolve unit cells with 100-150 Å edges and collect data to ~1-8 Å dmin from crystals of 0.01-0.1 mm3 volume. We are currently planning a time-of-flight quasi-Laue diffractometer 156 m from the target using a ~1.8 Å wavelength band centered at 2.4 Å. We will also provide relevant supporting facilities such a s perdeuteration and crystallization with appropriate access modes.

Date

18.06.2012

Time

14:45–15:45

Place

Physics HS3

Garching, Garching, Deutschland

Speaker

Dr. E. Oksanen, ESS, Lund, Sweden

Organizers

TUM/FRM II

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